An interactive bicycle simulator with six degrees of freedom motion system could bring the rider a very realistic riding feeling. An important component of the simulator is the full bicycle dynamic model that simulated the two-wheeled bicycle dynamics. It consists of two slightly coupled submodels: The stability submodel and the vibration submodel. The stability submodel solves the stability of the bicycle under rider’s active maneuvers and the vibration submodel evaluates the vibration response of the bicycle due to uneven road surface. The model was validated by several experiments and successfully applied to the interactive bicycle simulator.